Scientist - II

Education Requirements:

• Minimum: MS in biology, biochemistry, or related scientific field with 1–2 years of experience.
• Or: BS in biology, biochemistry, or related scientific field with 3 or more years of relevant post-graduation work experience in a scientific setting (industrial experience preferred).

Required Skills:

• Solid foundation in sterile, aseptic technique and sterile cell culture.
• Experience working in a biosafety cabinet (BSC) for sterile cell culture.
• Experience with mammalian cell culture, including passaging cells, cell counting, determining cell density, and dilutions.
• Experience with recombinant protein expression by transient transfection of mammalian cells.
• Proficiency in protein characterization techniques including SDS-PAGE, Western blot analysis, and UV-VIS spectroscopy.
• Detailed electronic notebook recordkeeping and meticulous maintenance of lab consumable and DNA inventories required.
• Excellent organization and communication skills.
• Ability to work both independently and as part of a team.

Preferred Skills:

• Experience with suspension mammalian cell cultures.
• Experience with large-scale (up to 10L) cell culture and bioreactor technology, including WAVE bioreactor systems.
• Experience with cell culture analyzers, including the BioProfile FLEX analyzer.
• Experience developing optimized expression conditions for hard-to-express proteins, including multimeric proteins.

Position Overview:

• The successful candidate will join the Protein Expression and Purification team in the Department of Infectious Diseases and Vaccines (IDVax).
• This team generates critical protein reagents for internal IDVax programs in support of target discovery and validation in the early discovery space.
• The primary focus for this position will be large-scale (up to 10 L) expression of recombinant proteins and virus-like particles (VLPs) by transient transfection of suspension mammalian cells across multiple expression systems, including shake flask and WAVE bioreactor technology.
• The candidate will report to a lead scientist in the expression group and work closely and coordinate with the protein purification and characterization group.