Scientist - II

Summary
The Neuroimmunology team is seeking a motivated and technically skilled Scientist II with a strong background in CNS cell biology to join the team in Cambridge. This role focuses on supporting the study of disease-associated functions and phenotypes through culturing of stable cell lines and iPS-derived microglia. The scientist will collaborate across multidisciplinary teams in genetics, immunology, chemical biology, and pharmacology to identify and validate novel therapeutic targets for neurodegenerative diseases. This is an exciting opportunity to contribute to cutting-edge research in a dynamic and collaborative environment.

Responsibilities
• Culture stable cell lines, primary rodent neurons/glia, and iPS-derived CNS cell models (e.g., microglia, neurons).
• Develop and perform disease-relevant cell-based functional assays.
• Apply standard cell transfection techniques using lipid-based and electroporation methods.
• Perform high-content imaging, live cell imaging, and immunofluorescence staining.
• Acquire and analyze cellular images; apply quantitative image analysis.
• Conduct biochemical and molecular profiling including protein/RNA extraction and detection (e.g., Western blot, ELISA, qPCR).
• Carry out cellular functional studies such as phagocytosis and cell motility assessments.
• Document and communicate experimental data clearly to cross-functional teams.
• Contribute to method optimization, troubleshooting, and data interpretation using critical thinking.

Education
• BS/BA in Biology, Neuroscience, Molecular Biology, Bioengineering, Biotechnology, or a related field (required)

Experience
• Minimum 2 years of relevant industry or academic experience
• Experience with sterile techniques and cell culture of immortalized lines, rodent neurons, and glia
• Practical knowledge of statistics and data analysis
• Experience in microscopy and image acquisition
• Familiarity with mouse neuroanatomy

Additional Information
• Preferred experience includes high-content imaging microscopy, live cell imaging, quantitative image analysis, and molecular biology techniques
• Strong communication and collaboration skills are essential
• Location: Cambridge, MA
• Full-time, on-site role
• Cross-functional collaboration is a key part of this role