Associate Scientist, Cell Based Assays

We’re seeking a hands-on scientist to execute high-throughput cell-based assays in 384-well format. You will culture and plate cells, dispense compounds using the Echo, run diverse detection readouts, and analyze dose–response data to deliver high-quality EC50 results for discovery programs.

Key Responsibilities:

• Maintain, expand, and plate mammalian cell lines for 384-well assays
• Set up and execute assays with compound dispensing via Labcyte/Beckman Echo
• Run and optimize detection formats (e.g., luminescence, cAMP, HTRF/TR-FRET, fluorescence)
• Generate dose–response curves (4-parameter logistic fits) and report EC50/IC50 with QC
• Troubleshoot assay performance, optimize Z’ factors, and document SOPs/results
• Collaborate with discovery teams to prioritize studies and timelines

Requirements:

• BS/MS in Biology, Pharmacology, Biochemistry, or related field; 2+ years industry or equivalent lab experience
• Proficiency in mammalian cell culture and 384-well assay workflows
• Hands-on experience with Echo acoustic dispensing
• Experience with plate readers and assay platforms (e.g., cAMP, HTRF, luminescent assays)
• Strong data analysis skills: 4PL curve fitting, EC50/IC50 determination, and curve QC (e.g., R^2, top/bottom constraints)
• Detail-oriented, organized, and comfortable in a fast-paced, high-throughput environment

Nice to Have:

• Experience with automation/LIQ handlers, LIMS/ELN, and statistical QC (Z’, CV, S/B)
• Familiarity with GraphPad Prism, ActivityBase, or equivalent analysis tools
• Hands-on experience with Echo acoustic dispensing